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Figure 5 | BMC Microbiology

Figure 5

From: Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus

Figure 5

Effects of doxycycline on hygromycin sensitivity of a strain expressing rtTA in conjunction with the tetO7-hph reporter gene. Ten thousand conidia were spotted onto the center of a plate containing 750 μg/ml hygromycin and the indicated concentrations of doxycycline (μg/ml) and colony diameter was measured with time. Below: levels of hph RNA levels in the rtTA transformant by Northern blot analysis. Total RNA isolated from overnight cultures grown in the presence of 0, 5 or 50 μg/ml of doxycycline was fractionated by agarose gel electrophoresis, transferred to nylon membranes, and probed with a 32P-labeled hph probe.

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