Table 1 Bacterial strains and plasmids used in this study.
Strains and plasmids | Relevant genotype or phenotype | Reference or source |
|---|---|---|
Strains | Â | Â |
B. subtilis 168 | wild type | BGSCa, 1A1 |
E. coli | Â | Â |
RB791 | F' [lacIq L8] hsdR+ hsdM | [32] |
UE26 | F- ptsG2 ptsM1 glk7 rpsL150 | [33] |
Plasmids | Â | Â |
pMD496 | pQE9 derivative carrying glcK fused in frame to the His tag-coding region | [6] |
pLM-GlcK [D22K] | pMD496 derivative carrying glcKD10K | This study |
pLM-GlcK [C178A] | pMD496 derivative carrying glcKC166A | This study |
pLM-GlcK [C187A] | pMD496 derivative carrying glcKC175A | This study |
pLM-GlcK [C189A] | pMD496 derivative carrying glcKC177A | This study |
pLM-GlcK [C194A] | pMD496 derivative carrying glcKC182A | This study |
pLM-GlcK [C294A] | pMD496 derivative carrying glcKC282A | This study |
pLM-GlcK [C333A] | pMD496 derivative carrying glcKC321A | This study |