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Table 1 Bacterial strains and plasmids used in this study.

From: Bacillus subtilis GlcK activity requires cysteines within a motif that discriminates microbial glucokinases into two lineages

Strains and plasmids Relevant genotype or phenotype Reference or source
Strains   
B. subtilis 168 wild type BGSCa, 1A1
E. coli   
RB791 F' [lacIq L8] hsdR+ hsdM [32]
UE26 F- ptsG2 ptsM1 glk7 rpsL150 [33]
Plasmids   
pMD496 pQE9 derivative carrying glcK fused in frame to the His tag-coding region [6]
pLM-GlcK [D22K] pMD496 derivative carrying glcKD10K This study
pLM-GlcK [C178A] pMD496 derivative carrying glcKC166A This study
pLM-GlcK [C187A] pMD496 derivative carrying glcKC175A This study
pLM-GlcK [C189A] pMD496 derivative carrying glcKC177A This study
pLM-GlcK [C194A] pMD496 derivative carrying glcKC182A This study
pLM-GlcK [C294A] pMD496 derivative carrying glcKC282A This study
pLM-GlcK [C333A] pMD496 derivative carrying glcKC321A This study
  1. a BGSC, Bacillus Genetic Stock Center, Ohio State University, Columbus, Ohio