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Figure 5 | BMC Microbiology

Figure 5

From: Bacillus subtilis GlcK activity requires cysteines within a motif that discriminates microbial glucokinases into two lineages

Figure 5

Fluorescence emission spectra of B. subtilis GlcK, GlcKC166A, GlcKC282A, and GlcKC321A, which were measured at excitation wavelength 280 nm (A), 295 nm (B), and subtraction of 280–295 nm. Samples (2.0 μM) were measured at 22°C in 50 mM Tris-Cl, pH 7.5. The spectra have been corrected by the buffer values. Experiments had been repeated three times.

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