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Table 4 Strain List

From: Allele specific synthetic lethality between priC and dnaAts alleles at the permissive temperature of 30°C in E. coli K-12

Strain priC dnaA dnaC Relevant Genotype Source or Derivation
ALO454 + 46 + tnaA300::Tn10 Kirsten Skarstad
AQ12251 + + + rnhA339::cat T. Kogoma
CAG18442 + + + thr-34::Tn10 [38]
CM740 + 5 +   Kirsten Skarstad
CM2556 + 167 +   Kirsten Skarstad
CM2733 + 602 +   Kirsten Skarstad
JC12390 + 508 + tnaA300::Tn10 Lab stock
JC13509a + + +   [18]
JC19008 + + 809 priA2::kan [28]
JC19165 303 + +   [18]
JC19257 + + 809,820 priA2::kan [18]
SS145 303 + +   JC19165 -> JC13509b
SS1091 + + 809   JC19008 -> SS1213c
SS1092 + + 809,820   JC19257 -> SS1213c
SS1099 303 + 809   JC19165 -> SS1091b
SS1100 303 + 809,820   JC19165 -> SS1092b
SS1201 + 508 + tnaA300::Tn10 JC12390 -> JC13509e
SS1213 + + + thr-34::Tn10 CAG18442 -> JC13509d
SS1504 303 508 809,820 tnaA300::Tn10 JC12390 -> SS1100e
SS1531 303 + + rnhA339::cat AQ12251 -> SS145
SS1543 303 508 + rnhA339::cat JC12390 -> SS1531f
SS1738 + + + tnaA300::Tn10 Lab Stock
SS1750 + 5 + tnaA300::Tn10 SS1738 -> CM740e
SS1751 + 167 + tnaA300::Tn10 SS1738 -> CM2556e
SS1752 + 602 + tnaA300::Tn10 SS1738 -> CM2733e
SS1793 303 167 + tnaA300::Tn10 SS1751 -> SS145e
SS1796 303 602 + tnaA300::Tn10 SS1752 -> SS145e
SS1797 303 5 + tnaA300::Tn10 SS1750 -> SS145e
SS1798 303 204 + tnaA300::Tn10 WM433 -> SS145e
SS3032 303 46 + rnhA339::cat ALO454 -> SS1531f
WM433 + 204 + tnaA300::Tn10 Mark Sutton
  1. aJC13509 has the following genotype: sulB103 lacMS286 Φ 80dIIlacBK1 argE3 his-4 thi-1 xyl-5 mtl-1 SmR T6R. The lacMS286 Φ 80dIIlacBK1 code for two partial copies of the lac operon [39, 40].
  2. bSelect for kanamycin resistance on minimal media.
  3. cSelect for threonine prototrophy on minimal media. Screen for the presence of the dnaC mutation by the method described in [18].
  4. dSelect for tetracycline resistance on rich media and screen for threonine auxotrophy.
  5. eSelect for tetracycline resistance on rich media at 30°C and then screen for temperature sensitive growth at 42°C.
  6. fSelect for tetracycline resistance on rich media at 30°C and then screen for restriction site polymorphism and or backcross to test the presence of the temperature sensitive allele.