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Table 1 dnaA alleles used in this work and some phenotypes

From: Allele specific synthetic lethality between priC and dnaAts alleles at the permissive temperature of 30°C in E. coli K-12

Allele

Amino Acid Change

Domain Affecteda

Ability to replicate P+ plasmids at 30°Cb

Ability to grow in priC303::kan at 30°C

dnaA46

A184V

H252Y

III

III

No

No

dnaA508

P28L

T80I

I

I

No

No

dnaA5

A184V

G426S

III

IV

Yes

Yes

dnaA167

V157E

III

Yes

Yes

dnaA204

I389N

IV

No

Yes

dnaA602

A184V

A347V

III

linker

Yes

Yes

  1. aDomains are defined according to [25, 35, 36].
  2. bData from [34]