Figure 1From: DNA-free RNA preparations from mycobacteriaAnalysis of DNA contaminations in a RNA preparation from M. smegmatis by RT-PCR . One-tenth of the volume after RT-PCR was loaded on a 1% agarose gel, which was stained with ethidium bromide. Three different samples for RT-PCR were used: an untreated RNA (indicated as w/o), a DNaseI treated preparation (DNaseI) and a sonicated and DNaseI treated extraction (sonic + DNaseI). The four gels show samples taken after cycle 23, 25, 27 and 30 of the PCR. For every RT-PCR a negative control (-) was performed, in which the RNA was added to the sample after the RT step.Back to article page