From: A simulation model of *Escherichia coli* osmoregulatory switch using E-CELL system

Reaction | Equation | Constant | Comments |
---|---|---|---|

Formation of EnvZk and EnvZp | v = k [envz] [s] v = k [envz] [s] | k = 15.6 μM k = 0.15 μM | (High osmolarity) (low osmolarity) Catalysed Mass action reactor, velocity is calculated as a product of concentrations of substrates and kinetic constants [Data as presented by Yoshida et al [29]] |

Formation of EnvZkp and EnvZpp | KmS = 1 μM* KcF = 10 μM* KmS = 10 μM* KcF = 100 μM* | As per chemotaxis data. Kinetics of the reaction described by Henri Michaelis Menten equation derived from rapid equilibrium assumptions [(Bray et al [4]] | |

Formation of EnvZkpompr and EnvZppompr complex | KmS = 0.51 μM* KcF = 10 μM* KmS = 0.42 μM* KcF = 20 μM* | As per chemotaxis data Kinetics of the reaction described by Henri Michaelis Menten equation derived from rapid equilibrium assumptions. [(Bray et al [4]] | |

Dissociation of EnvZkpompr, EnvZppompr, EnvZkomprp and EnvZpomprp complexes | v = rate | Rate = 1.20 μM | Represented by zero reactor, velocity is independent of concentration of molecular species [data as presented by Yoshida et al [29]] |

Formation of F1omprp, F1F2omprp, F1F2F3omprp | v = k [f1] v = k [f1f2] v = k [f1f2f3] v = k [f1f2f3f4] | k = 6.8 nM k = 10.7 nM k = 15.4 nM k = 21.2 nM | Mass action reactor, velocity is calculated as a product of concentrations of substrates and kinetic constants [data as presented Head et al [28]] |

Formation of C1omprp, C1C2omprp, C1C2C3omprp | v = k [c1] v = k [c1c2] v = k [c1c2c3] | k = 7.7 μM k = 18.9 μM k = 31.4 μM | Mass action reactor, velocity is calculated as a product of concentrations of substrates and kinetic constants [data as presented Head et al [28]] |

Degradation of F1F2F3F4 OmpRp (OmpF repression) | [f1f2f3f4omprp] | In the reactor class decay process, substrate reduced according to the half-life inputted. [Bergstrom et al [28]] | |

Formation of OmpC and OmpF | v(OmpC) = k [c1c2c3omprp] [envzk] v(OmpF) = k [f1f2f3fomprp] [envzp] | k = 1 μM k = 1 μM | Catalysed Mass action reactor, velocity is calculated as a product of concentrations of substrates and kinetic constants (Batchelor and Goulian [39]) |