From: A simulation model of Escherichia coli osmoregulatory switch using ECELL system
Reaction  Equation  Constant  Comments 

Formation of EnvZk and EnvZp 
v = k [envz] [s] v = k [envz] [s] 
k = 15.6 μM k = 0.15 μM 
(High osmolarity) (low osmolarity) Catalysed Mass action reactor, velocity is calculated as a product of concentrations of substrates and kinetic constants [Data as presented by Yoshida et al [29]] 
Formation of EnvZkp and EnvZpp 

KmS = 1 μM* KcF = 10 μM* KmS = 10 μM* KcF = 100 μM* 
As per chemotaxis data. Kinetics of the reaction described by Henri Michaelis Menten equation derived from rapid equilibrium assumptions [(Bray et al [4]] 
Formation of EnvZkpompr and EnvZppompr complex 

KmS = 0.51 μM* KcF = 10 μM* KmS = 0.42 μM* KcF = 20 μM*  As per chemotaxis data Kinetics of the reaction described by Henri Michaelis Menten equation derived from rapid equilibrium assumptions. [(Bray et al [4]] 
Dissociation of EnvZkpompr, EnvZppompr, EnvZkomprp and EnvZpomprp complexes  v = rate  Rate = 1.20 μM  Represented by zero reactor, velocity is independent of concentration of molecular species [data as presented by Yoshida et al [29]] 
Formation of F1omprp, F1F2omprp, F1F2F3omprp 
v = k [f1] v = k [f1f2] v = k [f1f2f3] v = k [f1f2f3f4] 
k = 6.8 nM k = 10.7 nM k = 15.4 nM k = 21.2 nM  Mass action reactor, velocity is calculated as a product of concentrations of substrates and kinetic constants [data as presented Head et al [28]] 
Formation of C1omprp, C1C2omprp, C1C2C3omprp 
v = k [c1] v = k [c1c2] v = k [c1c2c3] 
k = 7.7 μM k = 18.9 μM k = 31.4 μM  Mass action reactor, velocity is calculated as a product of concentrations of substrates and kinetic constants [data as presented Head et al [28]] 
Degradation of F1F2F3F4 OmpRp (OmpF repression)  [f1f2f3f4omprp]  In the reactor class decay process, substrate reduced according to the halflife inputted. [Bergstrom et al [28]]  
Formation of OmpC and OmpF 
v(OmpC) = k [c1c2c3omprp] [envzk] v(OmpF) = k [f1f2f3fomprp] [envzp] 
k = 1 μM k = 1 μM  Catalysed Mass action reactor, velocity is calculated as a product of concentrations of substrates and kinetic constants (Batchelor and Goulian [39]) 