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Table 2 Bacterial strains used in the study and results of the PCR identification system by Keyser et al. [12] in comparison to the system established in this study

From: 16S rRNA gene based analysis of Enterobacter sakazakii strains from different sources and development of a PCR assay for identification

No of strains

species

origin

PCR result (primers from this study)

PCR results (Keyser primer set)

1

E. sakazakii

ATCC 29004

+

+

1

E. sakazakii

ATCC 51329

+

negativ

1

E. sakazakii

ATCC 29544

+

+

10

E. sakazakii

fruit powder

+

+

1

E. sakazakii

milk powder

+

+

2

E. sakazakii

baby food

+

+

1

E. sakazakii

milk

+

+

16

E. sakazakii

production environment

+

+

14

E. sakazakii

human

+

+

1

E. cloacae

LMG 2783

-

-

1

E. cloacae

LMG 3008

-

-

1

E. cloacae

food

-

-

1

E. cloacae

food

-

positive

1

E. cloacae

clinical

-

-

1

P. dissolvens

LMG 2683

-

-

1

P. agglomerans

LMG 1286

-

-

3

P. agglomerans

food, clinical, cosmetics

-

-

1

E. hermanii

wild strain

-

-

1

E. coli

ATCC 25922

-

-

1

E. coli

wild strain

-

-

1

S. liquefaciens

cosmetics

-

positive

1

S. fucaria

production environment

-

positive

1

K. oxytoca

cheese

-

-

1

K. pneumoniae

wild strain

-

-

1

P. mirabilis

DSM 788

-

-

1

S. sonnei

ATCC 29930

-

-

1

S. Enteritidis

wild strain

-

positive

1

P. aeruginosa

ATCC 15442

-

-

1

L. acidophilus

ATCC 13651

-

-

1

S. aureus

ATCC 25923

-

-

1

S. agalactiae

ATCC 33019

-

-

1

B. cereus

ATCC 10876

-

-

1

E. faecium

DSM 2918

-

-

1

L. monocytogenes

wild strain

-

-

1

M. luteus

ATCC 9341

-

-