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Table 2 Bacterial strains used in the study and results of the PCR identification system by Keyser et al. [12] in comparison to the system established in this study

From: 16S rRNA gene based analysis of Enterobacter sakazakii strains from different sources and development of a PCR assay for identification

No of strains species origin PCR result (primers from this study) PCR results (Keyser primer set)
1 E. sakazakii ATCC 29004 + +
1 E. sakazakii ATCC 51329 + negativ
1 E. sakazakii ATCC 29544 + +
10 E. sakazakii fruit powder + +
1 E. sakazakii milk powder + +
2 E. sakazakii baby food + +
1 E. sakazakii milk + +
16 E. sakazakii production environment + +
14 E. sakazakii human + +
1 E. cloacae LMG 2783 - -
1 E. cloacae LMG 3008 - -
1 E. cloacae food - -
1 E. cloacae food - positive
1 E. cloacae clinical - -
1 P. dissolvens LMG 2683 - -
1 P. agglomerans LMG 1286 - -
3 P. agglomerans food, clinical, cosmetics - -
1 E. hermanii wild strain - -
1 E. coli ATCC 25922 - -
1 E. coli wild strain - -
1 S. liquefaciens cosmetics - positive
1 S. fucaria production environment - positive
1 K. oxytoca cheese - -
1 K. pneumoniae wild strain - -
1 P. mirabilis DSM 788 - -
1 S. sonnei ATCC 29930 - -
1 S. Enteritidis wild strain - positive
1 P. aeruginosa ATCC 15442 - -
1 L. acidophilus ATCC 13651 - -
1 S. aureus ATCC 25923 - -
1 S. agalactiae ATCC 33019 - -
1 B. cereus ATCC 10876 - -
1 E. faecium DSM 2918 - -
1 L. monocytogenes wild strain - -
1 M. luteus ATCC 9341 - -