Induction of protease activity in V. anguillarum wild type (circles) and vanT mutant strains (squares). Cells were grown for 16 h in LB20, harvested by centrifugation, washed twice in NSS, and resuspended at 2 × 109 CFU/ml in either LB20 (open symbols) or NSSM (solid symbols). (A) NB10 and vanT mutant, NB02. (B) M93Sm and vanT mutant, M02. Both of the experiments shown represent a single experiment, although each experiment was repeated at least three times with similar results.