Figure 4From: Gel shift analysis of the empA promoter region in Vibrio anguillarumInduction of protease activity in V. anguillarum NB10 (A) and M93Sm (B) cells incubated in LB20 (●), NSSM (■), 3M (▲) or NSS (▼). Cells were grown for 16 h in LB20 at 27°C, harvested by centrifugation, washed twice in NSS, and resuspended at 2 × 109 CFU/ml in each condition. Samples were taken at the indicated times and cell-free supernatant was tested for protease activity using the azocasein assay. Both of the experiments shown represent a single experiment, although each experiment was repeated at least three times with similar results.Back to article page