Figure 3From: Gel shift analysis of the empA promoter region in Vibrio anguillarumGel shift analysis of proteins from NB10 cells incubated in 3M and NSS. Cells grown overnight in LB20 were washed twice in NSS and resuspended at 2 × 109 cfu/ml in the appropriate medium. Protein extract (5 μg) from cells incubated for 3 h in each condition was added to the gel shift reaction with the DIG-labeled oligomer. DIG-labeled DNA alone was added to the lane marked with an asterisk (*). Reactions containing protein extracts prepared from each condition was loaded onto the gel: lane 1, NSSM; lane 2, NSSM + unlabeled oligomer; lane 3, LB20; lane 4, 3M; lane 5, NSS; lane 6, exponential phase cells in LB20; and lane 7, M93Sm in NSSM. The data presented are representative of three replicate experiments.Back to article page