Gel shift analysis of proteins from NB10 cells incubated in 3M and NSS. Cells grown overnight in LB20 were washed twice in NSS and resuspended at 2 × 109 cfu/ml in the appropriate medium. Protein extract (5 μg) from cells incubated for 3 h in each condition was added to the gel shift reaction with the DIG-labeled oligomer. DIG-labeled DNA alone was added to the lane marked with an asterisk (*). Reactions containing protein extracts prepared from each condition was loaded onto the gel: lane 1, NSSM; lane 2, NSSM + unlabeled oligomer; lane 3, LB20; lane 4, 3M; lane 5, NSS; lane 6, exponential phase cells in LB20; and lane 7, M93Sm in NSSM. The data presented are representative of three replicate experiments.