Figure 2

Gel shift analysis using a DIG-labeled 50 bp oligomer (0.8 ng/lane) containing the empA promoter region and putative lux box. Protein extracts were prepared from V. anguillarum M93Sm (left) and NB10 (right) incubated in (A) LB20 or (B) NSSM and added to DIG-labeled DNA. DIG-labeled DNA alone was added to the lane marked with an asterisk (*). Lane 1, 5 μg of protein extract from cells at time 0 h; lane 2, 5 μg protein extract from cells at time 3 h; lane 3, 7.5 μg protein extract from cells at time 3 h; lane 4, 5 μg protein extract from cells at time 3 h plus the unlabeled lux box-empA promoter containing oligomer; and lane 5, 5 μg protein extract from cells at time 3 h plus non-competitive oct2A (from E. coli) unlabeled 39 bp oligomer. The data presented are representative of three replicate experiments.