Table 2 Plasmids and primers used in this study

pUFR034

IncW Nm^r Mob⁺ mob (P) lacZ alpha Par⁺ cos (8.7 kb)

[44]

Pbluescript

Ap^r

Stratagene

pSD5

pUFR034 + a 35 kb insert from the BXO1 genome

[8]

pBP1

pBluescript + a 2.2 kb Eco RI fragment from pSR1

This study

pBP2

pBluescript + a 3.5 kb Eco RI fragment from pSR1

This study

pBP3

pBluescript + a 4.0 kb Eco RI fragment from pSR1

This study

pBP4

pBluescript + a 9.0 kb Eco RI fragment from pSR1

This study

pBP5

pBluescript + a 10 kb Eco RI fragment from pSR1

This study

Primers specific to ORFs present in the genomic island of BXO1

WxoA

Forward primer CCAAGCGACCAGAGGTGCTTGACG

Reverse primer GAGGAGCACCATCCGCTACCGCCC

WxoB

Forward primer GTTTTTGTTGGTACTGGGTGCGAG

Reverse primer GTACGCCACGGTCAGATCGGCTGC

WxoC

Forward primer CTACTGATGTTGTTCGCAAGGTGG

Reverse primer GGCGACTCACCTGCATATCGAGCC

WxoD

Forward primer GTGCTGGTGAGCCATCATTTTG

Reverse primer TTACTCACCGGCCATAATCCTTTT

Wzt

Forward primer GACATCGCTATCGAAGTAAAAGGT

Reverse primer TCAGGTGCTGTTTGAAGTAGCGGAC

Wzm

Forward primer CATCGGCAAACCCCTTTCGGGT

Reverse primer CGCAGCTTACTGATGGAACCCT

Primers used for TAIL PCR

designed against metB gene sequence of BXO1

CglL1

CTTCGACGCAGCCAAGCGTTTC

CglL2

CTGCGAGAAGACCGAGCTGTTCAC

CglL3

CGAATCGCTCGGTGGTGTTGAA

designed against etfA gene sequence of BXO1

EtfL1

TCGGCCAGACCGGCAAGATCAT

EtfL2

AGCTGTACATGGCCATCGGCAT

EtfL3

AGCATCTGACCGGCATCAAGGA

Other primers described in the paper

Pbp1

AGCGTGCTGGTGAGCCATCA

Pbp2

GCAGCAAAAATGCTGTCATAACCA