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Table 3 In vitro growth inhibition of M tuberculosis and M. marinum by aminooxy compounds. One hundred μL of a mid-logarithmic culture of M. tuberculosis or M. marinum at a concentration of 2 × 105 cfu/ml was added to 100 μL of serial doubling dilutions of inhibitor in a 96-well microtitre plate. The drug plates were grown for 14 days at 37°C (M. tuberculosis) or 8 days at 30°C (M. marinum) with no agitation before checking for cell growth at A650 nm. The minimum inhibitory concentration (MIC) and inhibitory concentration 50% (IC50) were calculated as described in the Methods section.

From: Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis

Inhibitor M. marinum (n = 8) M. tuberculosis (n = 6)
  MIC (mM) IC50 (μM) MIC (mM) IC50 (μM)
O-allylhydroxylamine 0.078 8.49 ± 1.96 0.156 39.22 ± 2.01
carboxymethoxylamine 0.313 89.32 ± 7.65 0.313 70.99 ± 7.83
O-benzylhydroxylamine 1.25 410.76 ± 67.10 1.25 467.54 ± 62.80
canaline 1.25 335.29 ± 13.64 1.25 273.18 ± 14.64
O-(t-butyl)hydroxylamine 10 43.18 ± 10.51 10 130.52 ± 23.10