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Table 2 Ki determination for selected aminooxy inhibitors. The enzyme was incubated with variable amounts of leucine and inhibitor and fixed amounts of KMTB, as described in the Methods section. Kic and Kiu refer to the competitive and uncompetitive components of mixed-type inhibition [25].

From: Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis

Inhibitor Kic(μM) Kiu(μM)
O-(t-butyl)hydroxylamine 11.02 ± 2.76 85.60 ± 44.79
carboxymethoxylamine 20.97 ± 7.27 142.42 ± 69.76
O-allylhydroxylamine 21.61 ± 11.08 >200 (386)*
O-benzylhydroxylamine 8.20 ± 2.56 84.08 ± 31.91
  1. *200 μM was the highest concentration of inhibitor tested in these experiments. The calculated Kiu value is shown in parentheses.