Skip to main content

Table 1 Kinetic characterization of M. tuberculosis branched-chain aminotransferase. The enzyme was incubated with varying concentrations of substrate and 10 mM cosubstrate, as described in the Methods section.

From: Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis

Substrate Cosubstrate Apparent Km (mM) Apparent Vmax (μmol/min/mg protein)
Leu KMTB 2.50 ± 0.90 3.65 ± 0.43
Val KMTB 1.77 ± 0.86 2.58 ± 0.41
Ile KMTB 2.85 ± 0.56 4.28 ± 0.32
Glu KMTB 9.53 ± 3.43 5.70 ± 1.20
Phe KMTB 7.44 ± 1.40 2.17 ± 0.22
KMTB Leu 4.20 ± 1.79 4.22 ± 0.72
Leu KG 6.02 ± 0.94 13.44 ± 0.84
Val KG 5.79 ± 0.99 11.82 ± 0.80
Ile KG 6.16 ± 1.14 14.35 ± 1.08
KG Leu 6.95 ± 1.44 12.80 ± 1.12