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Figure 1 | BMC Microbiology

Figure 1

From: Prediction of DtxR regulon: Identification of binding sites and operons controlled by Diphtheria toxin repressor in Corynebacterium diphtheriae

Figure 1

IdeR binds the predicted DtxR-binding DNA fragments. 30 pmoles of IdeR was added to 32P-labelled DNA probes in the presence of 200 μM Mn2+, and complexes were resolved on a 7% Tris-borate polyacrylamide gel containing 150 μM Mn2+. Lane 1: Control gel retardation using Radiolabeled DNA motif without DtxR-binding site. Lane 2: Radiolabeled DIP2161 motif without IdeR. Lane 3: Radiolabeled DIP2161 motif with IdeR. Lane 4: Radiolabeled DIP0699 motif with IdeR. Lane 5: Radiolabeled DIP0586 motif with IdeR. Lane 6: Radiolabeled DIP2304 motif with IdeR. Lane 7: Radiolabeled DIP2272 motif with IdeR.

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BMC Microbiology

ISSN: 1471-2180

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