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Table 3 Reproducibility of the LightCycler-PCR

From: Development of real-time PCR for detection of Mycoplasma hominis

Copy number No. of PCR runs where fluorescence gave positive signal CP SD CV % of CP
105 10 / 10 25.4 1.7 6.7
104 10 / 10 28.7 1.6 5.5
103 10 / 10 32.3 1.6 4.8
102 10 / 10 35.8 1.7 4.8
101 10 / 10 39.7 2.1 5.3
5 copies 10 / 10 40.6 2.3 5.6
1 copy 6 / 10 43.8 1.7 3.8
  1. CP = crossing-points, mean value
  2. CV = coefficient of variation
  3. SD = standard deviation
  4. Statistical analysis of standard dilution series of standard PG21 DNA was performed. Ten different runs were analysed for crossing-points values and for presence of fluorescence signal. Based on crossing-points values the standard deviation of single concentration was calculated and coefficient of variation as a function: CV% = (SD / Average CP value for each concentration) × 100%.