Table 2 Primers and probes sets used in the study
From: Development of real-time PCR for detection of Mycoplasma hominis
Oligonucleotide | Nucleotide position | Sequence |
|---|---|---|
Forward Primer | 295–322 | 5'-GGAAGA-TATGTAACAAAAGAAGGTGCTG-3' |
Reverse Primer I | 411–438 | 5'-TTTATCTTCTGGCGTAATGATATCTTCG-3' |
Reverse Primer II | 430–457 | 5'- ATGAAGCGCCTGATAAAATTTTATCTTC-3' |
Probe I | 336–368 | 5'-AGCAGGTGCTAAAAAGGTGTTTATTACTGCTCC-FL-3' |
Probe II | 370–408 | 5'-LCred705-GCTAAAAGCGAAGGTGTTAAAACAGTT GTTTATTCAGTA-3' |