Orientation of primers and probes with sequence comparison to the closer related genital bacteria M. genitalium. The DNA sequence of primers and probes from M hominis PG21 is compared to the homologous M. genitalium sequence. Eight mismatches were present in the forward primer, 7 in the first probe, 15 in the second probe and 13 in the reverse primer I. Highlighted nucleotides correspond to mismatches. Letters in bold correspond to the beginnings and ends of designed primers (forward and reverse I) and probes (I, II). Letters in italic correspond to beginning and ending of reverse primer II. The active site region of GAPDH enzyme is underlined.