Plasmid | Description | Reference |
---|---|---|
pBluescript | Cloning vector, ApR | Stratagene |
pDD515 | RSF1010 ori; a mobilizable broad host range vector, CmR | 12 |
pDD559 | pSR658 with vapD Hi in frame with wild-type LexA DNA-binding domain (DBD) | This work |
pDD560 | pTrcHisA with vapD Hi | This work |
pDD561 | pSR659 with vapD Hi in frame with mutant LexA DBD | This work |
pDD562 | pBluescript with vapD Hi region | This work |
pDD563 | pDD652 with an aph(3')II gene interrupting vapD Hi | This work |
pDD564 | pDD515 with a full-length vapD Hi region for transcomplementation | This work |
pDD577 | pSR658 with a truncated vapD Hi in frame with the wild-type LexA DBD | This work |
pDD594 | pDD515 with a truncated vapD Hi region for transcomplementation | This work |
pDD672 | pDD515 with the vapX Hi antitoxin for rescue | This work |
pSR658 | Wild-type LexA DBD fusion vector, TcR | 15 |
pSR659 | Mutant LexA DBD fusion vector, ApR | 15 |
pTrcHisA | Cloning vector, ApR | Invitrogen |
Strain | Description | Source |
DD12 | Conjugal host strain | 12 |
DH5α | Cloning strain | Promega |
SU101 | Homodimerization reporter strain | 14 |
SU202 | Heterodimerization reporter strain | 14 |