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Table 1 Plasmids and E. coli strains used in this study.

From: Identification and characterization of a nontypeable Haemophilus influenzae putative toxin-antitoxin locus

Plasmid Description Reference
pBluescript Cloning vector, ApR Stratagene
pDD515 RSF1010 ori; a mobilizable broad host range vector, CmR 12
pDD559 pSR658 with vapD Hi in frame with wild-type LexA DNA-binding domain (DBD) This work
pDD560 pTrcHisA with vapD Hi This work
pDD561 pSR659 with vapD Hi in frame with mutant LexA DBD This work
pDD562 pBluescript with vapD Hi region This work
pDD563 pDD652 with an aph(3')II gene interrupting vapD Hi This work
pDD564 pDD515 with a full-length vapD Hi region for transcomplementation This work
pDD577 pSR658 with a truncated vapD Hi in frame with the wild-type LexA DBD This work
pDD594 pDD515 with a truncated vapD Hi region for transcomplementation This work
pDD672 pDD515 with the vapX Hi antitoxin for rescue This work
pSR658 Wild-type LexA DBD fusion vector, TcR 15
pSR659 Mutant LexA DBD fusion vector, ApR 15
pTrcHisA Cloning vector, ApR Invitrogen
Strain Description Source
DD12 Conjugal host strain 12
DH5α Cloning strain Promega
SU101 Homodimerization reporter strain 14
SU202 Heterodimerization reporter strain 14