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Table 1 Plasmids and E. coli strains used in this study.

From: Identification and characterization of a nontypeable Haemophilus influenzae putative toxin-antitoxin locus

Plasmid

Description

Reference

pBluescript

Cloning vector, ApR

Stratagene

pDD515

RSF1010 ori; a mobilizable broad host range vector, CmR

12

pDD559

pSR658 with vapD Hi in frame with wild-type LexA DNA-binding domain (DBD)

This work

pDD560

pTrcHisA with vapD Hi

This work

pDD561

pSR659 with vapD Hi in frame with mutant LexA DBD

This work

pDD562

pBluescript with vapD Hi region

This work

pDD563

pDD652 with an aph(3')II gene interrupting vapD Hi

This work

pDD564

pDD515 with a full-length vapD Hi region for transcomplementation

This work

pDD577

pSR658 with a truncated vapD Hi in frame with the wild-type LexA DBD

This work

pDD594

pDD515 with a truncated vapD Hi region for transcomplementation

This work

pDD672

pDD515 with the vapX Hi antitoxin for rescue

This work

pSR658

Wild-type LexA DBD fusion vector, TcR

15

pSR659

Mutant LexA DBD fusion vector, ApR

15

pTrcHisA

Cloning vector, ApR

Invitrogen

Strain

Description

Source

DD12

Conjugal host strain

12

DH5α

Cloning strain

Promega

SU101

Homodimerization reporter strain

14

SU202

Heterodimerization reporter strain

14