Table 3 Details of virus stocks, cell lines and BEI inactivated arbovirus antigens
Virus Stock | Strain | Cell Line | Incubation (days/°C) | HA upon Harvest (titre/pH) | Inactivation Time (Hours) | Antigen Method |
|---|---|---|---|---|---|---|
RRV C6/36 | T48 | Vero | 3/37 | 2048/5.8 | 8 | CFF/SDGC |
BFV C6/36 | BH2193 | Vero | 3/37 | 2048/5.8 | 8 | CFF/SDGC |
CHIKV MBP | BKM459 | C6/36 | 2/28 | 1024/5.8 | 8 | CFF |
JEV PS-EK | Nakayama | PS-EK | 3/37 | 2048/6.2 | 5 | CFF/SDGC |
KUNV C6/36 | MRM16 | PS-EK | 3/37 | 1024/6.4 | 5 | CFF |
MVEV C6/36 | MRM66 | PS-EK | 3/37 | 2048/6.4 | 8 | CFF/SDGC |
ALFV C6/36 | MRM3926 | PS-EK | 3/37 | 512/6.4 | 5 | CFF |
DENV-1 C6/36 | TIM00 | C6/36 | 13/28 | 256/6.2 | 5 | CFF |
DENV-2 C6/36 | TIM00 | C6/36 | 8/28 | 256/6.2 | 8 | CFF/SDGC |
DENV-3 C6/36 | TIM00 | C6/36 | 7/28 | 512/6.2 | 5 | CFF/SDGC |
DENV-4 C6/36 | TIM00 | C6/36 | 11/28 | 256/6.2 | 5 | CFF/SDGC |
WNV C6/36 | Sarafend | PS-EK | 2/37 | 1024/6.2 | 5 | CFF |
YFV C6/36 | 17D | PS-EK | 3/37 | 128/6.4 | 5 | CFF |