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Figure 2 | BMC Microbiology

Figure 2

From: Yersinia enterocolitica type III secretion: Evidence for the ability to transport proteins that are folded prior to secretion

Figure 2

Native gel electrophoresis reveals binding of methotrexate (MTX) to YopE1–53-DHFR. Cytosolic fractions from yersiniae expressing YopE1–53-DHFR were prepared as described in legend to Fig. 1. Cytosolic fraction was loaded directly on a HEPES-buffered native gel (lane 1) and after further incubation with 1 mM MTX (lane 2). After electrophoresis gel was electroblotted and DHFR-fusions were detected using anti-DHFR antibodies.

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BMC Microbiology

ISSN: 1471-2180

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