Rapid identification and mapping of insertion sequences in Escherichia coli genomes using vectorette PCR

BMC Microbiology

Table 2 Primers used for identification of IS s using vectorette PCR

Primer Name^a	Sequence (5' to 3')^b	Length (bp)^c
IS1-A	gttacgcacc accccgtcag ta	22
IS1-B	cggaagtcgc tgtcgttctc aa	22
IS2-A	ggccc taagacatca atcatctg	23
IS2-B	tcgctcg ccacgggaat atctg	22
IS3-A	agccg ctgcgggcca cccggagcac	25
IS3-B	ggcct cagtccggaa caatttga	23
IS4-A	cgagagatgag ttcggggtcg agg	24
IS4-B	aagggccttc ccgagagtgg taa	23
IS5-A	gccatggca gaatctgctc catgcggg	27
IS5-B	tgtttcgggc ggaccaaatg ata	23
IS30-A	ccagctcgt atctcctcgc gctctg	26
IS30-B	ctagatctgg ttgctgctca gc	22
IS150-A	cctgacctgg gttcggggga cac	23
IS150-B	gcgaactgaa ggatgctgtt ac	22
IS186-A	gggccagaat tgctgaccag ttat	24
IS186-B	acctgaac tcgcgaaagc gtggata	25

^aPrimers are named after the insertion sequence with A and B designating each side. ^bSequences obtained from MG1655 [17]. ^cLength of primer in base pairs.

ISSN: 1471-2180