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Table 1 IS elements in closely related E. coli strains MG1655, CGSC6300, TD2 and TD10

From: Rapid identification and mapping of insertion sequences in Escherichia coli genomes using vectorette PCR

      A-side B-side Strain
Element start (bp) end (bp) Flanking Genes Direction fragment sizea fragment sizea MG1655 CGSC6300 TD2 TD10
Known Elements           
IS1-1 19811 20508 b0020-b0023 - 272 199* Pb P P P
IS1-2 278402 279099 b0263-b0266 - 174 191* P P P P
IS1-3 289873 290570 b0273-b0276 - 171* 577 P P P P
IS1-4 1049056 1049753 b0987-b0989 + 281* 139 P P P P
IS1-5 1976542 1977239 b1892-b1895 - 452 226 P P   
IS1-6 3581114 3581811 b3443-b3446 + 712 1020 P P P P
IS1-7 4516095 4516370 b4293-b4295 + 1354 575* P P P P
IS2-1 380530 381803 b0359-b0362 + 308 841 P P P P
IS2-2 1465945 1467218 b1401-b1405 - 194 203* P P P P
b1579 transposase 1649536 1650732 b1578-b1580 +   778 P P P P
IS2-3 2066974 2068247 b1995-b1998 - 287* 444 P P P P
IS2-4 2994394 2995622 b2859-b2862 - 589 219 P P P P
IS2-5 3184203 3185431 b3043-b3046 + 410 672 P P P P
IS2-6 4495795 4497068 b4271-b4274 + 204* 163 P P P P
IS3-1 390963 391829 b0371-b0373 - 489* 142 P P P P
IS3-2 566361 567227 b0540-b0542 + 787 346 P P P P
IS3-3 1093498 1094364 b1025-b1027 - 1009 772 P P P P
IS3-4 2168554 2169420 b2088-b2090 + 502 842 P P P P
IS3-5 314811 315677 b0298-b0300 + 863 158* P P P P
IS4 4499671 4500999 b4277-b4279 - 629 450 P P P P
IS5-1 273325 274341 b0258-b0260 - 489* 497 P P P P
IS5-2 573960 574976 b0551-b0553 - 590 1605 P P P P
IS5-3 687220 688236 b0655-b0657 - 348 420* P P P P
IS5-4 1394100 1395116 b1330-b1332 + 569* 471* (P)c (P) (P) (P)
IS5-5 1425770 1426750 b1369-b1371 - 491 1543 P P P P
IS5-6 2064327 2065343 b1993-b1995 - 747 1210 P P P P
IS5-7 2099917 2100933 b2029-b2031 - 306 303 P P P P
IS5-8 2287085 2288101 b2191-b2193 - 314* 411 P P P P
IS5-9 3128193 3129209 b2981-b2983 + 1085 672 P P P P
IS5-10 3363337 3364353 b3217-b3219 - 207* 1126 P P P P
IS5-11 3649812 3650828 b3504-b3506 - 734* 1335 P P P P
IS30-1 269827 270978 b0255-b0257 + 720 314 P P P P
IS30-2 1467382 1468533 b1401-b1405 + 305 297 P P P P
IS30-3 4505034 4506185 b4283-b4285 - 405 766 P P P P
IS 150 3718309 3719678 b3556-b3559 + 195 176 P P P P
IS186-1 15445 16557 b0015-b0017 + 503 1501 P P P P
IS186-2 607288 608400 b0581-b0583 + 2204 417 P P P P
IS186-3 2512345 2513463 b2393-b2395 + 764 667 P P P P
Additional Elements           
IS1-a 257908   b0240 - 1488 738   P   
IS1-b 335460   b0319-b0326 - 609 309     P
IS1-c 1871063   b1786 - 1211 484   P   
IS1-d 2037484   b1970-b1971 + 617* 1066    P P
IS1-e 2623548   b2502 + 282* 368    P P
IS1-f 2768501   b2635 + 1608 544*   P P P
IS1-g 3275070   b3130 + 529 2117    P P
IS1-h 4539642   b4313 - 175* 1119    P P
IS2-a 1588558   b1506 + 765 717    P P
IS2-b 2927499   b2796 + 138 368    P P
IS3-a 331175   b0314-b0315 + 1002* 563     P
IS3-b 838769   b0805 + 450 870*   P P P
IS3-c 2460317   b2351 + 489* 1642    P P
IS3-d 3427623   b3280 - 953 646    P P
IS3-e 3776882   b3604 - 1435 935*   P P P
IS3-f 4466303   b4242 - 640 935*   P P P
IS5-a 1102866   b1040-b1041 + 365 400    P P
IS30-a 4115565   b3927 - 692 186    P P
IS30-b 2246187   b2156 - 303* 445   P P P
IS186-a 4541184   b4314 - 606 534    P P
  1. a * indicates a fragment not recovered. b P, present. c Neither IS5-4 fragment is detected. Conventional PCR of gDNA confirms IS5-4 is present.
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BMC Microbiology

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