Figure 2
Bioassay for quorum sensing using C. violaceum CV026 demonstrating effects of compound 3.31 on violacein formation. Concentrations of compound 3.31 in rows A to F: Columns 1 to 3 = 10-2 M, columns 4 to 6 = 10-3 M, columns 7 to 9 = 10-4 M, columns 10 to 12 = 10-5 M. HHL concentrations: row A 10-6 M, row B 3.3*10-7 M, row C 1.1*10-7 M, row D 3.7*10-8 M, row E 1.2*10-8 M, row F 4.1*10-9 M. Rows G and H: calibration for HHL, G1-G3 10-6 M, G4-G6 10-7 M, G7-G9 10-8 M, G10-G12 10-9 M, H1-H3 10-10 M, H4-H6 10-11 M. H7-H9 is a growth control of the mutant CV026 lacking HHL, wells H10-H12 contain compound 3.31 at 10-2 M to observe growth inhibition.