Figure 5
From: Chlamydial development is blocked in host cells transfected with Chlamydophila caviae incA
Effect of cytosolic expression of deleted or mutated C. caviae incA sequences. Cells were transfected with either pCcAS17A, which encodes a protein differing from wild type at only a single amino acid (panel A), pCcAm2, which encodes a protein lacking the amino terminal 60 amino acids of IncA, (panel B), or pCcAM3, which encodes a protein lacking the carboxy terminal 116 amino acids of IncA (panel C). The fluorescence images are labeled with anti-polyhistidine monoclonal antibody (red), and anti-chlamydial HSP60 (green). The nuclei are labeled with DAPI (blue). The scale bar in C indicates 8 microns for each panel.