Figure 2

Effect of cytosolic expression of incA and incC from C. caviae or C. trachomatis. The vector pcDNA4/HisMaxC was used in each construct examined in this figure. IncA was detected by using specific monoclonal antibodies and labeled in red in each panel. Distribution of His/IncC fusion protein in the cytoplasm was detected indirectly by using anti polyhistidine tag monoclonal antibodies (Clontech) and also labeled in red. In panels B-F, the nuclei are labeled with DAPI (blue) and chlamydiae are labeled with anti-HSP60 (green). Panel A; HeLa cell transfected with pCcAWT (C. caviae incA). Panel B; HeLa cell transfected with pCcAWT in a monolayer subsequently infected at an MOI of 1.0 with C. caviae. Panel C; HeLa cell transfected with pCcAWT and subsequently infected at an MOI of 0.25 with C. trachomatis. Panel D; HeLa cell transfected with pCtAWT and subsequently infected at an MOI of 0.25 with C. trachomatis. Panel E; HeLa cell transfected with pCtAWT and subsequently infected at an MOI of 0.25 with C. caviae. Panel F; HeLa cell transfected with pCcCWT (C. caviae incC) and infected with C. caviae at MOI 1.0. The scale bar in F indicates 8 microns for each panel.