The outer membrane protein Omp35 affects the reduction of Fe(III), nitrate, and fumarate by Shewanella oneidensis MR-1

Table 2 Synthetic oligonucleotides used in this study.

Name	Oligonucleotide sequence^a
Oligonucleotides based on omp35
O1	5'-TGCTCGAG GGGCGGGTAAACGTAAACTCA-3'
O2	5'-TGCTCGAG CATGGCGCCTCACCTACCT-3'
O3	5'-ATTGCGATCGAT ATAACCGTTGGCGATCTT-3'
O4	5'-ATTGCGATCGAT TAACAAAGGTATTGCGGGTAT-3'
O5	5'-TTTAGAGAAGCGGTAGTCAGC-3'
O6	5'-ATCGCCAACGGTTATGAT-3'
O7	5'-ACGCCATATCGAGTTTACAT-3'
Oligonucleotide based on Km ^r gene
K1	5'-ATTGCGATCGAT TTATGCTTGTAAACCGTT-3'
Oligonucleotides based on cat gene
C1	5'-CAGACGGCATGATGAACCTGAATC-3'
C2	5'-CCACCGTTGATATATCCCAATGGC-3'

^aThe underlined regions indicate the following restriction endonuclease sites engineered into the oligonucleotides: Xho I sites in O1 and O2 and Cla I sites in O3, O4, and K1.

ISSN: 1471-2180