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Table 2 Synthetic oligonucleotides used in this study.

From: The outer membrane protein Omp35 affects the reduction of Fe(III), nitrate, and fumarate by Shewanella oneidensis MR-1

Name Oligonucleotide sequencea
Oligonucleotides based on omp35  
O1 5'-TGCTCGAG GGGCGGGTAAACGTAAACTCA-3'
O2 5'-TGCTCGAG CATGGCGCCTCACCTACCT-3'
O3 5'-ATTGCGATCGAT ATAACCGTTGGCGATCTT-3'
O4 5'-ATTGCGATCGAT TAACAAAGGTATTGCGGGTAT-3'
O5 5'-TTTAGAGAAGCGGTAGTCAGC-3'
O6 5'-ATCGCCAACGGTTATGAT-3'
O7 5'-ACGCCATATCGAGTTTACAT-3'
Oligonucleotide based on Km r gene  
K1 5'-ATTGCGATCGAT TTATGCTTGTAAACCGTT-3'
Oligonucleotides based on cat gene  
C1 5'-CAGACGGCATGATGAACCTGAATC-3'
C2 5'-CCACCGTTGATATATCCCAATGGC-3'
  1. aThe underlined regions indicate the following restriction endonuclease sites engineered into the oligonucleotides: Xho I sites in O1 and O2 and Cla I sites in O3, O4, and K1.