Western blot of lysed whole cells with an antibody specific for Omp35. Each lane was loaded with equivalent wet cell pellet weight (30 μg). The strains carrying the various plasmids are indicated above each lane. This blot is representative of duplicate experiments. The bars and numbers at the right indicate the migration and mass of the protein standards. The results shown are for aerobically grown cells. Analogous results were obtained with fumarate-grown cells (not shown).