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Table 3 Sensitivity of the detection tests performed onto the Light Cycler platform. For Smallpox virus, Monkey pox virus and Burkholderia species, native DNA was not available. For C. psittaci and Varicella-zoster virus titrated micro-organisms were not available.

From: Multi-pathogens sequence containing plasmids as positive controls for universal detection of potential agents of bioterrorism

Plasmid

Microorganism

Number of molecules detected

  

Plasmid

Native (strain)

CatA

Smallpox virus

5

NA

CatA

Y. pestis

5

5 (Kim5)

CatA

B. anthracis

5

5 (Sneath D455)

CatA

F. tularensis

500

5 (URFTMUE1)

CatB

Brucella sp.

50

50 (B. melitensis B115)

CatB

R. prowazeckii

50

100 (Brein l)

CatB

R. rickettsii

100

50 (VR 891)

CatB

B. mallei/pseudomallei

50

NA

CatB

C. burnetii

5

100 (Nine Mile)

CatB

C. psitacci

50

NA

CatVEF

Smallpox virus

5

NA

CatVEF

Monkeypoxvirus

5

NA

CatVEF

Orthopoxvirus

5

10 (ectromelia TC8)

CatVEF

Varicella-zoster virus

5

NA

CatVEF

Herpesvirus 1/2

5

10 (MA1)

CatVEF

R. akari

5

50 (Kaplan)