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Table 1 Genes and specific primers used for amplification of Bioterrorism agents. Bioterrorism agents are classified according to CDC recommendations. Their presence in the 3 constructed plasmids is given.

From: Multi-pathogens sequence containing plasmids as positive controls for universal detection of potential agents of bioterrorism

Agent

Priority Level

Targeted gene

Plasmid

Genbank access n°

Forward primer

Reverse primer

Bacteria

      

Bacillus anthracis

A

pag

CatA

AF065404

5'-AGGCTCGAACTGGAGTGAA-3'

5'-CCGCCTTTCTACCAGATTT-3'

Yersinia pestis

A

pla

CatA

M27820

5'-ATGGAGCTTATACCGGAAAC-3'

5'-GCGATACTGGCCTGCAAG-3'

Francisella tularensis

A

fop A

CatA

AF097542

5'-TAATAATTTCATTGCTCCTTTTG-3'

5'-TTCTATCTTGAGGACCCCAA-3'

Brucella spp.

B

omp 19

CatB

L27997

5'-CCGGTGAACTGGCTAATCT-3'

5'-TGAAGAATAGAGCGAGGCAA-3'

Burkholderia mallei

B

fl iC

CatB

AF11790

5'-GACGCTGGCGCTGTCGA-3'

5'-CGGCTTGTTGACCGCGTT-3'

B. pseudomallei

B

 

CatB

U82287

  

Chlamydia psittaci

B

omp A

CatB

AF269265

5'-ATTGCTTCGATTCAGATCAAC-3'

5'-ACCAGTGATTGACCATTTGT-3'

Coxiella burnetii

B

sod B

CatB

M74242

5'-CTTTTTACCGACTCCGCAAA-3'

5'-ACGAGCGTTGACAGTGCTT-3'

Rickettsia prowazekii

B

omp B

CatB

AF161079

5'-GCTCTTATGAATAATAATGCTC-3'

5'-TGTTGTAACGCAGCATTAACA-3'

Rickettsia rickettsii

B

omp A

CatB

U83451

5'-TTTGGTGGAGCTCATAAGTTA-3'

5'-GTACTATATTTGTTGTATTAAAAG-3'

Rickettsia akari

-

omp B

CatVEF

AF123707

5'-TTGGTGGGAATAATTGTAATGT-3'

5'-TGCAGCAAAATTGAATTCTG-3'

Viruses

      

Variola virus

A

Hemagglutinin

CatA, CatVEF

AF377893

5'-GACKTCSGGACCAATTACTA-3'

5'-TTGATTTAGTAGTGACAATTTCA-3'

Monkeypox virus

-

Hemagglutinin

CatVEF

AF375114

5'-AAAGTAGATTATGAAGAATACTC-3'

5'-CAGAAATAGTTTCGACAATTTCA-3'

Orthopoxvirus

-

Hemagglutinin

CatVEF

AF377892

5'-ACCAAGGATAAARTATCWTACG-3'

5'-ATAYATAAGTACCCGGCATCT-3'

Varicella- zoster virus

-

IE 62 transactivator

CatVEF

X04370

5'-GGTTAAACGTTTGAATCCATC-3'

5'-CAGCAGACTTTCTCGAACG-3'

Herpes simplex virus 1 and 2

-

DNA polymerase

CatVEF

X14112

5'-CCGACCCGGAGAGGGAC-3'

5'-CCAGGCGCTTGTTGGTGT-3'