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Table 1 Genes and specific primers used for amplification of Bioterrorism agents. Bioterrorism agents are classified according to CDC recommendations. Their presence in the 3 constructed plasmids is given.

From: Multi-pathogens sequence containing plasmids as positive controls for universal detection of potential agents of bioterrorism

Agent Priority Level Targeted gene Plasmid Genbank access n° Forward primer Reverse primer
Bacteria       
Bacillus anthracis A pag CatA AF065404 5'-AGGCTCGAACTGGAGTGAA-3' 5'-CCGCCTTTCTACCAGATTT-3'
Yersinia pestis A pla CatA M27820 5'-ATGGAGCTTATACCGGAAAC-3' 5'-GCGATACTGGCCTGCAAG-3'
Francisella tularensis A fop A CatA AF097542 5'-TAATAATTTCATTGCTCCTTTTG-3' 5'-TTCTATCTTGAGGACCCCAA-3'
Brucella spp. B omp 19 CatB L27997 5'-CCGGTGAACTGGCTAATCT-3' 5'-TGAAGAATAGAGCGAGGCAA-3'
Burkholderia mallei B fl iC CatB AF11790 5'-GACGCTGGCGCTGTCGA-3' 5'-CGGCTTGTTGACCGCGTT-3'
B. pseudomallei B   CatB U82287   
Chlamydia psittaci B omp A CatB AF269265 5'-ATTGCTTCGATTCAGATCAAC-3' 5'-ACCAGTGATTGACCATTTGT-3'
Coxiella burnetii B sod B CatB M74242 5'-CTTTTTACCGACTCCGCAAA-3' 5'-ACGAGCGTTGACAGTGCTT-3'
Rickettsia prowazekii B omp B CatB AF161079 5'-GCTCTTATGAATAATAATGCTC-3' 5'-TGTTGTAACGCAGCATTAACA-3'
Rickettsia rickettsii B omp A CatB U83451 5'-TTTGGTGGAGCTCATAAGTTA-3' 5'-GTACTATATTTGTTGTATTAAAAG-3'
Rickettsia akari - omp B CatVEF AF123707 5'-TTGGTGGGAATAATTGTAATGT-3' 5'-TGCAGCAAAATTGAATTCTG-3'
Viruses       
Variola virus A Hemagglutinin CatA, CatVEF AF377893 5'-GACKTCSGGACCAATTACTA-3' 5'-TTGATTTAGTAGTGACAATTTCA-3'
Monkeypox virus - Hemagglutinin CatVEF AF375114 5'-AAAGTAGATTATGAAGAATACTC-3' 5'-CAGAAATAGTTTCGACAATTTCA-3'
Orthopoxvirus - Hemagglutinin CatVEF AF377892 5'-ACCAAGGATAAARTATCWTACG-3' 5'-ATAYATAAGTACCCGGCATCT-3'
Varicella- zoster virus - IE 62 transactivator CatVEF X04370 5'-GGTTAAACGTTTGAATCCATC-3' 5'-CAGCAGACTTTCTCGAACG-3'
Herpes simplex virus 1 and 2 - DNA polymerase CatVEF X14112 5'-CCGACCCGGAGAGGGAC-3' 5'-CCAGGCGCTTGTTGGTGT-3'