Figure 2
From: The single-stranded DNA-binding protein of Deinococcus radiodurans
Expression and purification of the D. radiodurans R1 SSB protein. Protein expression was obtained from the pEAW328 vector in BL21 Codon Plus (DE3) (Stratagene) E. coli cells. Proteins were examined on a standard SDS-polyacrylamide gel. Lanes are (1) molecular weight markers (BioRad, Low Range), as noted with the labels at left; (2) Whole cell extract of uninduced cells; (3) whole cell extract of cells after induction of the D. radiodurans ssb gene; (4) purified D. radiodurans R1 SSB protein.