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Table 1 Cloning and culturea results for 8 healthy females with different grades of vaginal microflora.

From: Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis

Grade

I

I

I

II

II

III

III

III

Sample designation

BVS30

BVS62

BVS63

BVS36

BVS34

BVS59

BVS61

BVS44

Subject code

W1

W2

W3

W4

W5

W6

W7

W8

Age

51

34

38

49

41

46

28

44

Number of clones

124

118

107

69

72

125

169

70

Species

        

Lactobacillus crispatus [AF257097]b

66.1 c

 

99.1

     

Lactobacillus gasseri [AF243144]

18.5

99.2

      

Lactobacillus jensenii [AF243159]

  

0.9

     

Lactobacillus vaginalis [AF243177]

 

0.8

      

Atopobium vaginae [AF325325]

   

1.4

41.7

36.0

80.5

 

Atopobium vaginae 97.6%d [AF325325]

     

0.0

  

Gardnerella vaginalise [M58744]

   

0.0

 

0.0

4.1

 

Lactobacillus iners [Y16329]

   

84.1

1.4

 

12.4

 

Mobiluncus mulieris [AJ427625]

     

5.6

3.0

 

Peptostreptococcus anaerobius [L04168]

    

0.0

1.6

 

68.6

Peptoniphilus sp. [D14147]

     

0.0

 

14.3

Prevotella bivia 91% [L16475]

   

7.2

22.2

   

Prevotella buccalis 96.6% [L16476]

1.6

   

22.2

31.2

  

Sneathia (Leptotrichia) sanguinegens [L37789]

   

1.4

1.4

6.4

  

Uncultured Megasphaera sp. clone [AY271937]

   

1.4

 

4.8

  

Uncultured Actinobacteridae clone 86% [AB089070]

   

2.9

 

0.8

  

Unidentified clone 1f [AY207059]

    

6.9

4.0

  

Atopobium rimae [AF292371]

0.8

       

Fusobacterium nucleatum [AJ006964]

6.5

       

Peptostreptococcus sp. [AJ277208]

0.8

       

Pseudoramibacter alactolyticus [AB036761]

0.8

       

Streptococcus anginosus group [AF104676]

0.0

       

Treponema sp. clone [AF023055]

0.8

       

Porphyromonas levi clone 94% [L16493]

1.6

       

Unidentified clone 2 [AF371910]

2.4

       

Aerococcus christensenii [Y17318]

     

3.2

0.0

 

Anaerococcus tetradius [AF542234]

      

0.0

 

Anaerococcus vaginalis [AF542229]

    

1.4

   

Bacteroides ureolyticus [L04321]

      

0.0

 

Bifidobacterium biavatii (urinalis) [AJ278695]

       

1.4

Dialister sp. [AF473837]

       

2.9

Enterococcus faecalis [AJ420803]

    

1.4

   

[Leptotrichia amnionii] [AY078425]

    

1.4

   

Prevotella bivia [L16475]

   

0.0

0.0

   

Prevotella ruminicola 87% [L16476]

       

4.3

Streptococcus sp. oral strain [AY005041]

       

8.6

Unidentified clone 3 [AF371693]

     

6.4

  

Ureaplasma urealyticum [AF073455]

   

1.4

    
  1. a Four isolates were cultured which were negative for amplification of the 16S rRNA gene and for tDNA-PCR. b The accession number of the Genbank sequence with the highest similarity is indicated within brackets. c Underlined figures indicate that this species was also cultured. d Percentage of 16S rRNA gene sequence similarity, given only for values below 98%. e Low clone numbers of G. vaginalis are caused by the usage of one primer with 3 mismatches for this species (see Methods). f The 16S rRNA gene sequences of these clones showed highest similarity with entries in the Genbank listed as being obtained from uncultured bacteria.
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BMC Microbiology

ISSN: 1471-2180

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