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Table 1 Cloning and culturea results for 8 healthy females with different grades of vaginal microflora.

From: Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis

Grade I I I II II III III III
Sample designation BVS30 BVS62 BVS63 BVS36 BVS34 BVS59 BVS61 BVS44
Subject code W1 W2 W3 W4 W5 W6 W7 W8
Age 51 34 38 49 41 46 28 44
Number of clones 124 118 107 69 72 125 169 70
Species         
Lactobacillus crispatus [AF257097]b 66.1 c   99.1      
Lactobacillus gasseri [AF243144] 18.5 99.2       
Lactobacillus jensenii [AF243159]    0.9      
Lactobacillus vaginalis [AF243177]   0.8       
Atopobium vaginae [AF325325]     1.4 41.7 36.0 80.5  
Atopobium vaginae 97.6%d [AF325325]       0.0   
Gardnerella vaginalise [M58744]     0.0   0.0 4.1  
Lactobacillus iners [Y16329]     84.1 1.4   12.4  
Mobiluncus mulieris [AJ427625]       5.6 3.0  
Peptostreptococcus anaerobius [L04168]      0.0 1.6   68.6
Peptoniphilus sp. [D14147]       0.0   14.3
Prevotella bivia 91% [L16475]     7.2 22.2    
Prevotella buccalis 96.6% [L16476] 1.6     22.2 31.2   
Sneathia (Leptotrichia) sanguinegens [L37789]     1.4 1.4 6.4   
Uncultured Megasphaera sp. clone [AY271937]     1.4   4.8   
Uncultured Actinobacteridae clone 86% [AB089070]     2.9   0.8   
Unidentified clone 1f [AY207059]      6.9 4.0   
Atopobium rimae [AF292371] 0.8        
Fusobacterium nucleatum [AJ006964] 6.5        
Peptostreptococcus sp. [AJ277208] 0.8        
Pseudoramibacter alactolyticus [AB036761] 0.8        
Streptococcus anginosus group [AF104676] 0.0        
Treponema sp. clone [AF023055] 0.8        
Porphyromonas levi clone 94% [L16493] 1.6        
Unidentified clone 2 [AF371910] 2.4        
Aerococcus christensenii [Y17318]       3.2 0.0  
Anaerococcus tetradius [AF542234]        0.0  
Anaerococcus vaginalis [AF542229]      1.4    
Bacteroides ureolyticus [L04321]        0.0  
Bifidobacterium biavatii (urinalis) [AJ278695]         1.4
Dialister sp. [AF473837]         2.9
Enterococcus faecalis [AJ420803]      1.4    
[Leptotrichia amnionii] [AY078425]      1.4    
Prevotella bivia [L16475]     0.0 0.0    
Prevotella ruminicola 87% [L16476]         4.3
Streptococcus sp. oral strain [AY005041]         8.6
Unidentified clone 3 [AF371693]       6.4   
Ureaplasma urealyticum [AF073455]     1.4     
  1. a Four isolates were cultured which were negative for amplification of the 16S rRNA gene and for tDNA-PCR. b The accession number of the Genbank sequence with the highest similarity is indicated within brackets. c Underlined figures indicate that this species was also cultured. d Percentage of 16S rRNA gene sequence similarity, given only for values below 98%. e Low clone numbers of G. vaginalis are caused by the usage of one primer with 3 mismatches for this species (see Methods). f The 16S rRNA gene sequences of these clones showed highest similarity with entries in the Genbank listed as being obtained from uncultured bacteria.
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