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Table 3 Frequency of conjugal transfer of pB264 derivatives from Rhodococcus sp. B264-1 to Rhodococcus sp. I24.

From: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus

Plating Condition Description #CFU per ml
LB + gentamicin, 30°C Donors 3.51 ± 0.78 × 108
LB, 37°C Recipients 4.32 ± 0.98 × 109
LB + gentamicin, 37°C Transconjugantsa 3.17 ± 0.37 × 103
  1. a Several of the transconjugants were subsequently tested, and the presence of unrecombined pJANET was confirmed in each of these I24 derivatives.