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Table 3 Frequency of conjugal transfer of pB264 derivatives from Rhodococcus sp. B264-1 to Rhodococcus sp. I24.

From: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus

Plating Condition

Description

#CFU per ml

LB + gentamicin, 30°C

Donors

3.51 ± 0.78 × 108

LB, 37°C

Recipients

4.32 ± 0.98 × 109

LB + gentamicin, 37°C

Transconjugantsa

3.17 ± 0.37 × 103

  1. a Several of the transconjugants were subsequently tested, and the presence of unrecombined pJANET was confirmed in each of these I24 derivatives.