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Table 1 Key plasmids and strains used in this study.

From: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus

Bacterial Strains Description Source or Reference
   Escherichia coli XL1-Blue used for routine plasmid manipulation Stratagene, La Jolla, CA
   Rhodococcus sp. B264-1 Source of pB264; cannot grow above 32°C [13]
   Rhodococcus sp. B264-1 R1 spontaneous RifR-derivative of B264-1 this study
   Rhodococcus sp. I24 orange colonies; grows well at 37°C [13]
   Rhodococcus sp. I24 R6 RifR-derivative of I24 this study
   R. erythropolis SQ1 buff-colored colonies; RifR, StrR; derivative of ATCC4277 [8]
Plasmids   
   pAL220 pB264, ligated into pCR-Script this study
   pAL224 KanR marker from pUC4K ligated as an Eco RI fragment into the Eco RI site of pAL220 this study
   pAL231 TsrR marker from pGM160 ligated as an Eco RI-Sma I fragment into the Eco RI and Eco RV sites of pAL220 this study
   pAL281 GntR plasmid carrying NG2 origin for replication in Rhodococcus and E. coli this study
   pAL298 GntR marker from pGM160 ligated as an Eag I fragment into the Not I site (partial digest) of pAL220 this study
   pAL305 Pst I deletion derivative of pAL298 lacking majority of pB264 element this study
   pAL312 ORF7 from pB264 positioned downstream of constitutive trc promoter in a plasmid bearing the NG2 origin and a spectinomycin resistance marker this study
   pAL314 ORF7 and ORF8 from pB264 positioned downstream of constitutive trc promoter in a plasmid bearing the NG2 origin and a spectinomycin resistance marker this study
   pB264 4970 bp cryptic plasmid from Rhodococcus sp. B264-1 this study
   pCR-Script AmpR plasmid for cloning blunt-end DNAs Stratagene, La Jolla, CA
   pGM160 source of GntR and TsrR markers [35]
   pJANET majority of pB264 in vector carrying NG2 origin of replication and GntR this study
   pUC4K source of KanR Amersham Pharmacia, Piscataway, NJ
   pXS9a KanR marker from pUC4K ligated as an Eco RI fragment into the Eco RI site of pAL231 this study