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Table 2 Plasmids and primers used in this study

From: Expression of a novel gene, gluP, is essential for normal Bacillus subtilis cell division and contributes to glucose export

Plasmids

Relevant characteristics

Primers

Reference/source

pAC6

Vector for transcriptional lacZ fusions

 

[41]

pLR-P01

pAC6 Eco RI Bam HI; 2117 bp

5'atgccattttcgcggtttct3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatccgtcgccggttttatctgtcg3'd

 

pLR-P011

pAC6 Eco RI Bam HI; 2000 bp

5'ccggaattcgcggtaaacatgtttttgc3'

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatccgtcgccggttttatctgtcg3'd

 

pLR-P012

pAC6 Eco RI Bam HI; 1679 bp

5'ccggaattcgagaagggaactgtgtcag3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatccgtcgccggttttatctgtcg3'd

 

pLR-P013

pAC6 Eco RI Bam HI; 980 bp

5'ccggaattcggacatatcggcggcttga3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatccgtcgccggttttatctgtcg3'd

 

pLR-P014

pAC6 Eco RI Bam HI; 674 bp

5'ccggaattcgcgggtttcccttttggaac3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatccgtcgccggttttatctgtcg3'd

 

pLR-P015

pLR-PO1 derivative Bst BI;

-

this study

 

1313 bp fragmentb

  

pLR-P02

pAC6 Eco RI Bam HI; 352 bp

5'ccggaattcgcggtaaacatgtttttgc3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatccctgacacagttcccttctc3'd

 

pLR-P03

pAC6 Eco RI Bam HI; 730 bp

5'ccggaattcgagaagggaactgtgtcag3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatcctcaagccgccgatatgtcc3'd

 

pLR-P04

pAC6 Eco RI Bam HI; 346 bp

5'ccggaattcggacatatcggcggcttga3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatccgttccaaaagggaaaccgc3'd

 

pLR-P021

pAC6 Eco RI Bam HI; 469 bp

5'atgccattttcgcggtttct3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatccctgacacagttcccttctc3'd

 

pLR-P031

pAC6 Eco RI Bam HI; 1168 bp

5'atgccattttcgcggtttct3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatcctcaagccgccgatatgtcc3'd

 

pLR-P032

pAC6 Eco RI Bam HI; 1051 bp

5'ccggaattcgcggtaaacatgtttttgc3'c

this study

 

Eco RI-Bam HI fragmenta

5'cgcggatcctcaagccgccgatatgtcc3'd

 

pLR-YqgP

pRK415 Bam HI Kpn I; 1877-

5'cgcggatcgcgcggtttctgccgtcatgt3'c

this study

 

bpBam HI Kpn I fragmenta

5'cggggtacctttcctcaccatttcttg3'd

 

pLM-YqgP-Km1

pLR-YqgP derivative; gluP::aphA3 Kmr

-

this study

pRK415

Tcr, low-copy number plasmid

 

[46]

  1. aSize of the cloned PCR fragment after digestion with restriction enzyme as indicated bThe deletion within the 2112 bp DNA fragment of the pLR-P01, which is digested by Bst BI and religated fForward primer; dReverse primer