Figure 1From: Expression of a novel gene, gluP, is essential for normal Bacillus subtilis cell division and contributes to glucose exportTranscription of B. subtilis glucokinase operon. (A) Glucokinase operon is located between chromosomal 2569.795 and 2572.793 kb, as is available in the SubtiList database [12]. β-galactosidase assays of the transcriptional glucokinase operon – lacZ fusions were determined quantitatively as described in materials and methods. Average value of β-galactosidase activity (U/mg protein) is presented from two independent experiments. β-galactosidase activity (bluish green colour) was also monitored in vivo using LB plates containing X-Gal. (B) Northern blot of glucokinase transcript with a probe localized within glcK.Back to article page