Figure 2

A test array of the E. coli J96 genomic DNA (five different spotting concentrations with five replicates each) was hybridized with a Cy3 direct labeled 1 kb hly gene probe prepared with random priming (a), with a single stranded 1 kb hly gene fragment with a 5' capture sequence and detected by Cy3 DNA Dendrimer (b), and with fluorescein labeled 1 kb hly probe and detected with Tyramide Signal Amplification (TSA) system (c).