Table 1 Sequences and predicted lengths of PCR amplification products of oligonucleotide primers used
Target | Primer | Oligonucleotide sequence (5'-3')Sequence | Product size | Reference |
|---|---|---|---|---|
stx | VT1 | ATTGAGCAAAATAATTTATAT GTG | 523 bp | 21 |
VT2 | TGATGATGGCAATTCAGTAT | 520 bp | ||
eae | SK1 | CCCGAATTCCGCACAAGCATAAGC | 863 bp | 22 |
SK2 | CCCGGATCCGTCTCGCCAGTATTCG | |||
eae-α1 | EAE-FB | AAAACCGCGGAGATGACTTC | 820 bp | 7 |
EAE-A | CACTCTTCGCATCTTGAGCT | |||
eae-α2 | IH2498aF | AGACCTTAGGTACATTAAGTAAGC | 517 bp | 7 |
IH2498aR | TCCTGAGAAGAGGGTAATC | |||
eae-β1 | EA-B1-F | CGCCACTTAATGCCAGCG | 811 bp | 7 |
EAE-B | CTTGATACACCTGATGACTGT | |||
eae-β2 | EA-B2-F | CCCGCCACTTAATCGCACGT | 807 bp | 7 |
EAE-B | CTTGATACACCTGATGACTGT | |||
eae-γ1 | EAE-FB | AAAACCGCGGAGATGACTTC | 804 bp | 7 |
EAE-C1 | AGAACGCTGCTCACTAGATGTC | |||
eae-γ2/θ | EAE-FB | AAAACCGCGGAGATGACTTC | 808 bp | 7 |
EAE-C2 | CTGATATTTTATCAGCTTCA | |||
eae-δ/κ | EAE-FB | AAAACCGCGGAGATGACTTC | 833 bp | 7 |
EAE-D | CTTGATACACCCGATGGTAAC | |||
eae-ε | EAE-FB | AAAACCGCGGAGATGACTTC | 722 bp | 7 |
LP5 | AGCTCACTCGTAGATGACGGCAAGCG | |||
eae-ζ | Z1 | GGTAAGCCGTTATCTGCC | 206 bp | 7 |
Z2 | ATAGCAAGTGGGGTGAAG | |||
eae-η | EAE-FB | AAAACCGCGGAGATGACTTC | 712 bp | 7 |
LP8 | TAGATGACGGTAAGCGAC | |||
eae-ι | EAE-FB | AAAACCGCGGAGATGACTTC | 807 bp | 7 |
LP7 | TTTATCCTGCTCCGTTTGCT | |||
eae-λ | 68.4F | CGGTCAGCCTGTGAAGGGC | 466 bp | 7 |
68.4R | ATAGATGCCTCTTCCGGTATT | |||
eae-μ | FV373F | CAACGGTAAGTCTCAGACAC | 443 bp | 7 |
FV373R | CATAATAAGCTTTTTGGCCTACC | |||
eae-ν | IH1229aF | CACAGCTTACAATTGATAACA | 311 bp | 7 |
IH1229aR | CTCACTATAAGTCATACGACT | |||
eae-ξ | EAE-FB | AAAACCGCGGAGATGACTTC | 468 bp | 7 |
B49R | ACCACCTTTAGCAGTCAATTTG | |||
ehxA | HlyA1 | GGTGCAGCAGAAAAAGTTGTAG | 1551 bp | 23 |
HlyA2 | TCTCGCCTGATAGTGTTTGGTA | |||
ast A | EAST1 | CCA TCA ACA CAG TAT ATC CGA | 111 bp | 24 |
EAST2 | GGT CGC GAG TGA CGG CTT TGT | |||
EAF | EAF1 | CAG GGT AAA AGA AAG ATG ATA A | 397 bp | 25 |
EAF25 | TAT GGG GAC CAT GTA TTA TCA | |||
bfpA | EP1 | AAT GGT GCT TGC GCT TGC TGC | 326 bp | 26 |
EP2 | GCC GCT TTA TCC AAC CTG GTA |