Skip to main content

Advertisement

Table 1 msp2 hypervariable region primers to determine newly identified msp2 paralogs in low passage A. phagocytophilum genomic DNA.

From: Restricted changes in major surface protein-2 (msp2) transcription after prolonged in vitro passage of Anaplasma phagocytophilum

msp2 transcript locus Forward primer sequence (name) Reverse primer sequence (name)
msp2 (pan-msp2) 5'-TGATGTTGTTACTGGACAGA-3' (MSP465f) 5'-CACCTAACCTTCATAAGAA-3' (MSP980r)
RW1 5'-GCGACGAGGAAGGACAGTGGT-3' 5'-AGTCTTCAACCCAGTCCCGCT-3'
RW17 5'-GTGACGAAGAATTACGACAGT-3' 5'-GCTAGAAGATCCGGTTCCTGT-3'
RW20 5'-GTGACGAAGAAGGGGACAAAC-3' 5'-GTTAGAGGCTCCGGTTCCTGT-3'
RW23 5'-ACAAAGCGGAAGGATGGTGAT-3' 5'-ATTCGATTAGACGTTTCACT-3'