Profilin is required for viral morphogenesis, syncytium formation, and cell-specific stress fiber induction by respiratory syncytial virus

Table 1 Requirement of profilin for viral maturation

Host cell	Extracellular viral titer (Pfu/ml)
	Untreated	Profilin siRNA	Luciferase siRNA
HEp-2	3 × 10⁷	5 × 10⁴	2 × 10⁷
A549	2 × 10⁷	6 × 10⁴	1.5 × 10⁷

Monolayers of HEp-2 or A549 at low cell density (about 25% confluency) were infected with RSV Long at an m.o.i. of 3, in the presence or absence of the indicated siRNA (400 nM). The liberated virus was titered on HEp-2 cells using standard procedures. The coefficients have been rounded to the nearest integer and variations have been omitted for the sake of simplicity. Each number is an average of at least three experiments whereby variations never exceeded 20%.

ISSN: 1471-2180