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Table 4 Primers used in PCR and RT-PCR experiments performed in this study Primer sequences are shown 5' to 3'. "L" annotates sense, "R" antisense primers. In addition, the proposed gene names, as depicted in Fig. 1, and PCR product sizes are shown (see also Fig. 5).

From: Presence and expression of hydrogenase specific C-terminal endopeptidases in cyanobacteria

Synechocystis PCC 6803 Primer Sequence Gene Name Product Size [bp]
   Bidirectional hydrogenase    
hoxH6803/L AAT CCC ACG CCC TAA GTT TT   
   hoxH 200
hoxH6803/R CAC TGA CCA AGC AGA GTG GA   
hoxW6803/L TCA TCG GTT ACG GCA ATA CC   
   hoxW 198
hoxW6803/R ATG GTT CGT TTG CTG ATT CC   
Nostoc punctiforme ATCC 29133/PCC 73102    
   Uptake hydrogenase    
hupL-L CTG TTG GGC GGA CAA TGG CCT CA   
   hupL 383
hupL-R CCA CTT TTC ATA ATC AT   
hybD73102/L CGC AGG GAT GGA AGT AAT GT   
   hupW 218
hybD73102/R TCA CAT CAT CGG GAA AGT CA   
Anabaena PCC 7120    
   Uptake hydrogenase    
hupL7120/L2 TAT ATC ACC CGT CGG TAG AG   
   hupL 205
hupL7120/R2 CCA TGA TGC AGA GGT TAA GT   
hybD7120/L CGC AGG GAT GGA AGT TAT GT   
   hupW 209
hybD7120/R GCG GAA AAT CAT CTG GAA A   
   Bidirectional hydrogenase    
hoxHF CCA CTA TGC TCG TTT AAT TGA AAT   
   hoxH 355
hoxHR GGA ACA ACT TAA ACA GGG GTC AAA   
hoxW7120/L2 ATC TCT TGC AGT CCA TCA AC   
   hoxW 200
hoxW7120/R2 CGT AGA TGG CTT TGG TTA AG