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Figure 9 | BMC Microbiology

Figure 9

From: A novel Leishmania infantum nuclear phosphoprotein Lepp12 which stimulates IL1-beta synthesis in THP-1 transfectants

Figure 9

Enzymatic activity present in THP-1 cells phosphorylates recombinant protein Lepp12, in the presence of 10 microCi gamma-P32 ATP in an in vitro assay. E. coli lysate (cl, lane 1), reaction buffer (rb, lane 2) and GST (lane 4) were used as negative substrate controls. Phosphorylation of histone mix (h, lane 3) by THP-1 lysate (THP-1L) and of GST-Lepp12 (rLepp12) by exogenous PKC (ePKC, lane 5) were performed as positive controls. n = no enzymatic activity added. Autoradiogram is shown after exposition time of 24 h.

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