Figure 7
Recombinant protein Lepp12 is phosphorylated by an enzymatic activity present in promastigote lysate (proML), in the presence of 10 microCi gamma-P32 ATP in an in vitro assay. rLepp12: GST-Lepp12; h: histone mix as positive phosphorylation reaction control; GST; rb: reaction buffer; cl: E. coli lysate, as negative controls. n = no enzymatic activity added. Autoradiogram is shown after exposition time of 24 hours.