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Figure 2 | BMC Microbiology

Figure 2

From: A novel Leishmania infantum nuclear phosphoprotein Lepp12 which stimulates IL1-beta synthesis in THP-1 transfectants

Figure 2

Purification of recombinant proteins. The recombinant proteins GST-Lepp12 (rLepp12, 38.5 kDa, lane 1) and GST (29 kDa, lane 2) were purified from E. coli BL21 induced cultures (0.1 mM IPTG for 2 h). Bacteria were extracted with 1% Triton X-100 and fusion proteins were purified on gluthatione sepharose column. Five microg of eluted material was analyzed by SDS-PAGE (14% polyacrylamide) and Coomassie Blue staining. Molecular mass markers are indicated in kDa.

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BMC Microbiology

ISSN: 1471-2180

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