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Figure 3 | BMC Microbiology

Figure 3

From: Staphylococcus aureus - induced tumor necrosis factor - related apoptosis - inducing ligand expression mediates apoptosis and caspase-8 activation in infected osteoblasts

Figure 3

FACS analysis of normal mouse osteoblasts infected with S. aureus at a MOI of 75:1 in the presence or absence of anti-human TRAIL antibody (1.0 or 2.0 μg/ml). Following a 45-minute incubation, osteoblasts were washed 3 times with HBSS and incubated in medium containing 25 μg of gentamicin per ml to kill extracellular bacteria. Cells were stained with annexin V and propidium iodide 24 hours post-infection and analyzed using FACS. Results are presented from one osteoblast culture, and are representative of results from three different osteoblast preparations.

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