Table 1 Primer sequences for sequencing of mer A genes and for merA-TGGE.
mer primers | Direction | Nucleotide sequence (5' to 3') |
|---|---|---|
primers used for sequencing | ||
T1 | Forward | GTCTGGATCGGCAACTTGA |
P1 | Forward | GGCTATCCGTCCAGCGTCAA |
A1 | Forward | ACCATCGGCGGCACCTGCGT |
A5 | Reverse | ACCATCGTCAGGTAGGGGACCAA |
A7 | Reverse | TGGCGCATTGACAGTBGACCC |
A9 | Forward | GAAYTGGATCCAGACGGCKG |
A10 | Reverse | GATCATGATCTTGGACGGCACACA |
D1 | Reverse | TCATGGCAAACTCTCCGC |
TGGE primers (GC-clamp in italics) | ||
GC-merAf | Forward | CCCGCCGCCCCGCCCGCCGCCCCGCCCCGCCGCCCGCC TTGGAGAACGTGC |
merAr | Reverse | ACGTCCTTGGTGAAGGTCTG |