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Table 2 Substrate specificity of purified recombinant Ht-YgjG

From: Molecular cloning and characterization of Escherichia coli K12 ygjG gene

Substrate

Relative activity (%)a

Amino group donorb

 

Putrescine

100

Cadaverine

97

Spermidine

32

Agmatine

2

L-Ornithine

2

Amino group acceptorb

 

2-OG

100

α-ketobutyric acid

38

Pyruvic acid

12

  1. a The PATase activity with 2-OG as amino group acceptor was arbitrary defined as 100% relative activity. b 2-OG was used as amino acceptor with different polyamines and amino acid as amino group donors, and putrescine was used as amino group donor with different amino acceptors at final concentrations of 10 mM, respectively.