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Table 1 Expression of the lacZ fusions under different growth conditions

From: Molecular cloning and characterization of Escherichia coli K12 ygjG gene

 

β-Galactosidase activity, MUb

Plasmida

LBc

M9, Nitrogen Sufficientc

M9, Nitrogen Limitedc

pBR-σ54-lacZ-1

190

3,500

22,500

pBR-σ54-lacZ-2

140

400

800

pBR-lacZ-3

110

130

170

  1. a Plasmids were transformed into E. coli TG1 strain. b In Miller units [24]. c For growth conditions and β-galactosidase assay see Materials and Methods.